Another crucial time for protein is before bed. While sleeping the body releases a flood of anabolic hormones such as growth hormone and testosterone. This is yet another time when steps can be taken to preserve muscle while dieting. During the night we typically go several hours without eating. For this reason a slowly digesting protein would be best. This will allow a longer steady flow of amino acids that will continue to feed muscle tissue for hours.
The pre-training meal may just be the most important meal of the day. This is the meal that will fuel your workout. For this meal it is important to get protein and carbs which will make their way into the blood stream around the time your training session is getting underway. The glucose in the bloodstream from the carbs will be used for energy, while the amino acids from the protein will spare stored amino acids from being catabolized during training.
The simple act of performing cardio is extremely catabolic, because of this MISS sessions should be kept to a moderate duration. While HIIT sessions should be 10-20 minutes, MISS sessions should be in the range of 20-35 minutes. Once sessions go beyond this duration the cumulative effect of muscle tissue breakdown starts to become a serious concern.
This may explain why the fat-burning effects of eating more protein were confirmed in a study published in the American Journal of Physiology. One group was fed a high-protein diet (just over 1 gram per pound of body weight per day) while the second group consumed an amount closer to the lower recommendation of the RDA (recommended dietary allowance). The group eating the higher-protein diet burned the most fat.